#4536 INFLUENCE OF REDUCTION IN THE MASS OF ACTIVE NEPRONS ON THE EXPRESSION OF MIRNA AND NFκB IN THE MYOCARDIA AND AORT OF WKY RATS

Abstract Background and Aims At present, there is no doubt about the relationship between state of kidneys cardiovascular system, which reflected in concept cardiorenal continuum. The emerging data suggest that miRNAs may be one links connecting system. However, information role epigenomic changes development damage to heart blood vessels chronic kidney disease remains not fully understood. Target. aim work was evaluate expression levels microRNA-21, 133,203, as well nuclear transcription factor κB (NFκB) tissues myocardium aorta remodeling with a reduction mass active nephrons experiment. Method Systolic pressure (BP, mm Hg), myocardial index (MMI, mg/g), relative miRNA-21, -133, -203 NFκB were analyzed rats Wistar-Kyoto (WKY) 5/6 nephrectomy (Nx) duration experimental exposure 2 mo (n = 9). Controls sham operated (SO) animals reverse reaction for ‘complementary’ DNA (cDNA) preparation carried out under StemLoop-technology separately studied miRNA. following primers used PCR: microRNA-21–5'-GCCCGCTAGCTTATCAGACTGATG-3_, microRNA-133–5_-GCCCGCAGCTGGTAAAATGGAAC-3_, microRNA-203–5_-GCCGGTGAAATGTTTAGGACC-3_ U6–5_-GCGCGTCGTGAAGCGTTC-3_, common 5_-GTGCAGGGTCCGAGGT-3. semi-qualitative evaluation miRNA level (in units, RU) 2-(ΔΔCt) protocol at laboratory referent (0.09) calculations. quantified using StemLoop technology RealTimePCR compared reference-gene GAPDH. Data Student's t-test Mann–Whitney, P<0.05 considered statistically significant. Results In WKY an decrease nephron comparison control, significantly higher (median [interquartile rang]) (145.5[135.0; 155.0] vs 117.5[110.0; 120.0] Hg; P<0.001) (1.25[0.87; 1.87] 0.89[0.31; 1.15] units; P<0.01) (0.87[0.57;1.63] 0.35[0.22; 0.87] P<0.05). miRNA-21 increased NE (1.48[0.54; 3.73] 0.37[0.06; 1.62] SO group, but did change (p>0.05). Relative microRNA-133 both more than 10 times lower those group (p<0.001). BMI months after (p<0.05). Conclusion An number functioning only leads increase rats, also causes vessels, particular, activation signaling pathways associated rats. Possibly, it accompanied by growth NFκB-associated proliferative, proinflammatory profibrotic cytokines. It possible microRNA-21 involved formation fibrosis remodeling. specific mechanisms involvement pathogenesis vascular require further study.